The Yak 2.0 Genome Database is based on a female Leiwuqi-yak, through PacBio sequencing, Illumina sequencing, Bionano assembly, and Hi-C three-dimensional genome assistance, it has assembled the most complete detailed physical map of yaks at the chromosome level. It breaks through the problem that the genome cannot be assembled to the chromosome level due to the inability of large domestic or wild mammals to construct physical maps, which greatly prevents the precise positioning of this species in breeding, disease prevention and control, and other aspects. This result is several orders of magnitude higher than the integrity of the genome database that was previously fragmented by yak assembly, bringing yak and even livestock zoo research to a high level of accurate background information. BosGru-A total of 190527 gaps were found in the v2.0 version (the previously published version of the yak genome completed using second generation sequencing), with a total length of 118M. 165966 gaps were completely supplemented, accounting for 87.11%, and the length of the supplemented gap was 96.6M, accounting for 81.9%. Yak Genome 2.0 more completely covers the important functional gene regions of the genome, and more accurately assembles and annotates the repetitive sequences in the genome, truly completing the construction of the genome fine map of this species, laying a reliable and solid foundation for various subsequent studies of this species.